Algorithm for creating ILP markers for molecular genetic analysis of Triticum durum and other types of cereals

Aim. Development of an algorithm for creating ILP markers suitable breeding studies durum wheat (Triticum durum) and some other types cereals. Methods. Use classical bioinformatics tools to locate potential intron sites target genes in T. use as based on homology contigs rice (Oryza sativa) coding sequences. Analysis exon-intron structure homologous O. sativa, Hordeum vulgare, Aegilops tauschii Triricum aestivum followed by selection appropriate primers markers. Results. A number location introns contig sequences were identified. Degenerate designed them, taking into account the analysis corresponding H. A. aestivum. Conclusions. An creation was developed, which can be used molecular genetic plants case absence or incomplete sequenced genome studied species lack information about their genes. that cereals, particular durum, have been